Table 1.
Nomenclatures of RNAs used in this work
Name | Sequence |
---|---|
SDR1a | 5′ A1 UC, ACC, UCC, UUA12 |
bSDR1b | 5′ biotin-AUC, ACC, UCC, UUA |
bSDR1su7c | 5′ biotin-AUC, ACC, sU7CC, UUA |
SDR2a | 5′ U13AA, GGA, GGU, GAU24 |
bSDR2b | 5′ biotin-UAA, GGA, GGU, GAU |
SDR2-u18d | 5′ UAA, GGu, GGU, GAU |
See Fig. 1A for the numbering scheme.
Biotin attached to the 5′ phosphate via a (CH2)6 linker during chemical synthesis.
sU = 4-thio-uridine.
An adenine to uridine mutation (lower case letter) introduced at the 18th position.