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. Author manuscript; available in PMC: 2008 Jun 11.
Published in final edited form as: Circ Res. 2008 Mar 14;102(5):519–528. doi: 10.1161/CIRCRESAHA.107.168369

Figure 2.

Figure 2

Dilations in response to step increases in intraluminal flow (Panel A) or administration of acetylcholine (Panel B) in isolated, cannulated, first order gracilis muscle arterioles (d: ~100 μm; pressurized to 80 mmHg74) of aged (24 month old) F344 rats fed a standard diet (SD) are impaired, as compared to young vessels. Lifelong caloric restriction (CR) preserved microvascular endothelial function. *P<0.05 vs. aged SD. Data are mean±S.D. (n=4 in each group). Panel C: Original Western blots showing that expression of eNOS is up-regulated in carotid arteries of aged CR rats. Bar graphs (Panel D) are summary densitometry data. #P<0.05 vs. SD. Panel E: Lucigenin chemiluminescence measurements revealed that age-related increases in O2.- production in the aorta of F344 rats are prevented by lifelong caloric restriction (CR). *P<0.05 vs. young, #P<0.05 vs. standard diet (SD)-fed rats.