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. Author manuscript; available in PMC: 2009 Mar 1.
Published in final edited form as: Stem Cells. 2007 Nov 29;26(3):600–610. doi: 10.1634/stemcells.2007-0309

Figure 6.

Figure 6

WFU3 clone 3 cells have enhanced clonogenicity and an increased p63 to AR ratio. (A): Clonogenic assays. WFU3, WFU3 clone 3, and SR parent cells were inoculated on 60-mm dishes at the indicated densities and allowed to grow for 4 or 5 days prior to fixation and crystal violet staining as described in Materials and Methods. Left panel shows digital photos of representative dishes after staining. The mean number of colonies ± SD of quadruplicate dishes is depicted below each plate and in the graph at the right. In the graph, lines with the same letter next to them are not statistically significantly different from each other by analysis of variance; p ≤ .05 was considered significant. **, p ≤ .001. Numbers in parentheses are the average colony-forming efficiency ± SD of all inoculation densities, excluding 2,000 cells per dish. (B) Immunofluorescence microscopy of monolayer cultures of WFU3, WFU3 clone 3, and SR parent. Each top panel represents detection with a different primary antibody (indicated). Each bottom panel shows detection of 4,6-diamidino-2-phenylindole fluorescence from same area. Original magnification, ×60. (C): Immunoblot of protein lysates from the indicated cells probed for AR, p63, and β-actin. The numbers under each lane are the ratio of band intensity relative to actin loading. Abbreviations: AR, androgen receptor; CK, cytokeratin; ND, not determined.