Table 1.

Sequences of the Forward and Reverse Primers Used for the PCR Amplification of Phospholipase Isoforms in Human Corneal Epithelium

Phospholipase Isoform	Forward Primer	Reverse Primer	Expected PCR Product Size (bp)	GenBank Accession Number
sPLA2GIB	TGGTCATCTCAGTTCTTTTC	TCAACTCTGACAATACTTCT	482	NM_000928
sPLA2GIIA	AGCCACCAAGGAGGAGCAGG	CAGCACTGGGTGGAAGGTTT	309	NM_000300
sPLA2GIID	GCCAGCATCTGCCTCCACT	AGGAACAGGGTAGAGGGTGA	499	NM_012400
sPLA2GIIE	TGCTCCTTGTGCACCTC	CAGCTTGTTGGGATAATGGG	437	NM_014589
sPLA2GIIF	AGAAGTTCTTCACCGT	TTGGTGACTGCAGGTGACCT	480	NM_022819
sPLA2GIII*	TGCCTACAGAATCAGCACGA	TTGAGCAGCTGGAACTCGAT	500	AF_220490
sPLA2GV	TGGATACCAATGTTCCGAC	CCTAGGAGCAGAGGATGTTG	549	NM_000929
sPLA2GX	CGCCTATATGAAATATGGT	CAAGGTAGTCAGTCACACTTG	327	NM_003561
sPLA2GXII	AAGGAGGCGTGGATATGGAGCT	ATCTGTCACTAGCTGTCGGCAT	723	NM_030821
cPLA2α	GAGCTGAAAAAGGATCCTGACT	TGTCCCTAGAGTTTCATCCA	440	M68874
cPLA2β†	CTTCATGATGCCAGCTGAGCGCCGCC	CCCGGCCATCAGTGGGGCCTGCGC	2730	AF121908
cPLA2γ	CCACAGGCATCTATGTTGAA	ATTACTCTCTGACCGACTTC	1020	XM_055864
cPLA2δ	AATTATGGAGAGCCTGTCACCTGGG	TATATCAGGTCTGTGCCCATGGAGG	2457	AB090876
PLCβ1	TTGGATGTGGGGAACATCGG	AAATAGTGAGAAAGGGGCTG	690	XM_045636
PLCβ2	GAGCAACTAGATTTCTGGAG	TCACCGGAATCTTCCCTTCA	620	BC009009
PLCβ3	AGCGGTTCCTGAACAAGCTG	AGGTCTTGAAGGCAGTCTCG	550	NM_000932
PLCβ4	CCCAGTGGAAAGAATGATGA	TTCCATCCCAGCAGTCAAGT	500	L41349
PLCγ1	GCTGCCTGCGGATGGGCTGT	GTACCACTCTTTGCTCTCGT	924	NM_002660
PLCγ2	GCTGCCTGCGCATGGGCTGT	GTACCACGGCTTGGACTCGT	882	BC_011772
PLCδ1	TGTCGCTACTCAAGTGAGTC	ATGGAGCCTGAGTGGTGGAT	458	BC050382
PLCδ2	CAGGGTAGGTTACCTATTAA	ATGTTCGTAACAAAACCTAC	369	N/A‡
PLCδ3	AGCCATGCTGTGCGGCCGCT	AGACGGTCGTTGTTGGAGTG	716	NM_133373
PLCδ4	CAGCTCACAGACACAGGAAA	GCTGGTGACAACATCCACCA	468	NM_032726
PLCε§	CAGCAGAAGGTAATGGCT	TCCCTTGGGCTTTGGGAAAT	700	NM_016341 NM_015184
PLCζ1	GAGGGTATGCCAATTACACTT	GGCTGTTTTATTGCGATGCA	447	NM_033123

^*Degousee et al.³⁵

^†Pickard et al.³⁸

^‡Bovine sequence for PLCδ2 isoform, Meldrum et al.²³

^§Concensus sequence for PLCεisoforms.