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(a) MALDI mass spectrum of E. coli tRNATyrII digested with RNase A. (b) MALDI mass spectrum of the CMC-derivatized RNase A digest of E. coli tRNATyrII after alkaline treatment. Table 2 lists the RNase A digestion products including those which retain CMC after alkaline treatment along with tRNA signature digestion products identified from contaminating E. coli tRNAs in the sample.
