Table 2.
RNase A digestion products of E. coli tRNATyrII arising from data in Figure 3. Sequences listed are those that map to the known sequence of tRNATyrII or tRNA signature products [24] for contaminating E. coli tRNAs. ‘>p’ represent 2′-3′ cyclic phosphate digestion products. Modified nucleosides are defined in Table 1.
| tRNATyrII RNase A fragment | Calc m/z | (Fig. 3a)
Exp m/z |
(Fig. 3b)
After Reaction with CMC (n = # CMC units) |
|---|---|---|---|
| GGU>p | 995.1 | 995.1 | |
| G[Gm]C>p | 1008.1 | 1007.9 | |
| G[Gm]Cp | 1026.1 | 1026.1 | |
| GAAU>p | 1308.1 | 1308.0 | |
| AGACp | 1325.2 | 1324.9 | |
| A[ms2i6A]AΨ>p | 1406.0 | 1406.0 | 1657.6 (n = 1); 1909.9 (n = 2) |
| A[ms2i6A]AΨp | 1424.0 | 1424.0 | 1675.6 (n = 1); 1926.9 (n = 2) |
| GGGG[s4U]p | 1719.0 | 1718.8 | minor peak detected at 1970.9 |
| GAAGGm5Up | 2030.2 | 2029.8 | |
| AAAGGGAGCp | 3018.4 | 3018.4 | |
| RNase A Signature Ions (tRNA) | Calc m/z | (Fig. 3a)
Exp m/z |
(Fig. 3b)
After Reaction with CMC (n=#CMC units) |
|
| |||
| A[ms2i6A]AACp (Trp) | 1752.3 | 1751.9 | 2002.8 (n = 1) |
| AAAAGCp (Ser) | 1983.3 | 1982.8 | |
| GAGAGCp (Gly) | 2015.3 | 2014.8 | |
| GAAAGGGUp (Ser) | 2690.3 | 2690.6 | |