Table 3.
Summary of RNase digestion products containing ms2i6A. “n” depicts the number of CMC units bonded to the oligonucleotide. Modified nucleosides are defined in Table 1.
| Endonuclease | ms2i6A Sequence and tRNA | Control m/z | CMC-derivated m/z |
|---|---|---|---|
| RNase A | A[m2i6A]A¬p; tRNATyrII | 1424.0 | 1675.6 (n=1) |
| 1926.9 (n=2) | |||
| RNase T1 | ACUQUA[ms2i6A]A¬CUGp; tRNATyrII | 4097.6 | 4350.9 (n = 1) |
| 4601.0 (n = 2) | |||
| RNase A | A[ms2i6A]AACp; tRNATrp | 1751.9 | 2002.8 (n=1) |
| RNase T1 | U[Cm]UCCA[ms2i6A]AACCGp; tRNATrp | 3943.6 | 4195.6 (n=1) |
| RNase A | A[ms2i6A]AACp; tRNASer | 1751.9 | 2002.8 (n=1) |
| RNase T1 | A[ms2i6A]AACCGp; tRNASer | 2402.3 | 2653.8 (n=1) |