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Localization of endogenous Brd4 protein visualized by staining with anti-zebrafish Brd4 antibody. (A-A” and B-B”): pre-MBT (256-512 cell stage) embryos, (C-C” and D-D”): post-MBT (sphere to dome stage) zebrafish embryos. A-C, prophase. Scale bar: 10 μm.