Fig. 7.
Bromodomain-deleted zebrafish Brd4 proteins do not associate with mitotic chromosomes. Constructs are shown schematically at the top: Brd4, FLAG-tagged construct containing both bromodomains; constructs with deletions of bromodomain 1 (dBD1), bromodomain 2 (dBD2), or both bromodomains (dBD1 and 2). RNAs were injected into 1-2 cell stage zebrafish embryos, and stained at the sphere stage with anti-FLAG antibody (green). Arrows indicate mitotic chromosomes. Scale bar: μm.