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. 1997 Nov 25;94(24):12944–12948. doi: 10.1073/pnas.94.24.12944

Figure 1.

Figure 1

Hmg2p degradation required the UBC7 gene. (A) Stability of myc-Hmg2p in wild-type, ubc6::HIS3 (ubc6Δ), or ubc7::LEU2 (ubc7Δ) strains as measured by stationary chase. Equal numbers of cells in log phase (L) or early stationary phase (sφ) were analyzed by SDS/PAGE and anti-myc IB. A sample of the wild-type strain also was grown into stationary phase in the presence of lovastatin (25 μg/ml) to show the regulation of myc-Hmg2p degradation caused by slowing of the mevalonate pathway (Right). (B) Degradation of myc-Hmg2p by cycloheximide chase. Log-phase cultures of the indicated strains were treated with cycloheximide (50 μg/ml final). At the indicated times, equal numbers of cells were lysed and analyzed by IB.