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. 1997 Nov 25;94(24):12944–12948. doi: 10.1073/pnas.94.24.12944

Figure 5.

Figure 5

ZA stimulated degradation of Hmg2p-GFP in vivo. (A) Hmg2p-GFP degradation of Hmg2p-GFP (in strain RHY513) was examined in the absence (no drug) and presence (plus ZA) of ZA by methionine chase. In each panel, 20–25 cells are photographed at the indicated times with identical optical settings. (B) Effect of ZA on steady-state levels of Hmg2p-GFP. Early log phase cells expressing Hmg2p-GFP (RHY513) were grown for 3 hr in the presence of ZA, ZA plus HMG-CoA synthase inhibitor L-659,699 (Fig. 3), or no drug, and then analyzed by FACS with 10,000 cells per histogram. Horizontal axis is log of relative fluorescence.