Summative assessment of image interpretation, imaging tools, and biological scale. Histogram of responses to pre- and post- course assessments focusing on learning goals in which students were asked to (a) interpret images of healthy and unhealthy lungs (n = 34), (b) explain and give examples of imaging tools (n = 36), and (c) define biological scale (n = 35). The change in distributions between the pre- and postcourse assessment indicated that students made positive learning gains toward our learning goals, which were for students to understand the prominent place of quantitative imaging in medicine, to recognize the scope of imaging tools and their application to examine structure and function, and to understand the meaning of biological scale in imaging.