Figure 2.

In-class assessment of image quantification. Measurements of NanoBucky's height using ImageJ were reported as (a) micrometers (n = 3 for students; n = 10 for instructor) or (b) pixels (n = 10 for students; n = 10 for instructor). Students demonstrated proficiency in using imaging software because the height estimates in micrometers [t(11) = −0.52; p = 0.61; unpaired, 2-tailed] or pixels [t(18) = 1.56; p = 0.14; unpaired, 2-tailed] were not significantly different between the students and instructor. This satisfied our learning goal for students to recognize imaging as a quantitative tool.