Table 1.

Alignment of misconceptions, learning goals, learning outcomes, and activities

MisconceptionsStudents mistakenly think that:
-Biological science and imaging science are unrelated	-Biological images can only reveal structure but not function	-Biological images are pretty pictures but not quantifiable	-The impact of imaging techniques on biology and medicine is limited
Learning GoalsStudents will:
-Conclude that the scale of biology studied must match the scale captured by an imaging device	-Appraise the utility of imaging tools to examine structure and function of biological processes	-Discover that imaging is a quantitative tool used to measure data across a wide range of biological scale	-Generalize that biological images hold prominent places in advancing biology and medicine and contribute to the NIH Roadmap initiative
-Develop interpretation skills by examining different imaging formats and multiple biological scales using real data	-Evaluate imaging methods that provide ways of knowing structure and function	-Develop computer and computation skills	-Understand that imaging is an integrated multidisciplinary field
Learning OutcomeHaving achieved the learning goal, students will be able to:
-Summarize the relationship between biological and imaging scale	-To predict the rabbit Alba's color from fluorescence emission of eGFP	-Proficiently use NIH ImageJ software to calculate NanoBucky's height and image intensity	-Value that (1) nanoimaging advances the development of nanodevices and nanomedicine
-Accurately interpret:(1) nanoimages of NanoBucky (2) molecular images of fluorescent probe intensity from (a) bovine pulmonary artery endothelial cells (b) eGFP labeled human embryonic stem (ES) cells (3) system-level images of (a) eGFP rabbit Alba (b) MRI image of brain (c) CT image of Phineas Gage's skull	-Evaluate the intensity of eGFP labeled ES cells to understand ES function	-Apply ImageJ principles to: (1) Interpret the intensity of eGFP labeled cells	(2) molecular imaging advances tissue engineering and regenerative medicine
	-Assess functional brain activity using 18-FDG PET measures of glucose metabolism	(2) Quantify and localize brain function using PET images	(3) system-level imaging (e.g., PET) advances nuclear medicine
	-To explain and give examples of imaging tools		-Identify disease using X-ray images of lung with and without pneumonia and PET images of brain with and without a tumor
-Compare 2D, 3D VR, and stereolithograph models of brain and Phineas Gage's skull and contrast them for visual information quality and biological utility

Activities Students engage in the following activities to achieve the goals:

Mini-lectures, ImageJ analysis, in-class NIH Roadmap activities, Gillespie assessment, SALG assessment, pre and postinstruction assessment