Figure 2.

The absence of Hsp90 function does not affect the solubility of most of newly synthesized yeast proteins. Cells grown to mid-logarithmic phase at 25°C were labeled with [³⁵S]methionine at 25°C, 34°C, or 36°C for 45 min and maintained at the same temperatures for an additional hour in unlabeled medium. Solubility of proteins was assessed in wild-type (W) and hsp90^G170D (M) cells by centrifugation of cell lysates at 340,000 × g, followed by SDS/PAGE of total lysates (L), supernatants (S), and pellets (P). Proteins were visualized by autoradiography.