Figure 3.

The absence of Hsp90 function has little effect on the heat-induced inactivation of test substrates. Wild-type (□) and hsp90^G170D (⋄) cells expressing firefly luciferase (A) or bacterial luciferase-V.h.MAV (B) were grown to mid-logarithmic phase in galactose at 25°C to allow the accumulation of active test substrates. Cells were then shifted to the indicated temperatures for 15 min and the extent of enzyme inactivation was determined immediately. Enzyme activities were normalized to the activity present in each strain prior to heat inactivation. Data are the means and standard deviations of three independent experiments using three independent transformants.