Figure 1.

Neuroblastoma cells expressing native neurofascin segregate in the presence of neuroblastoma cells expressing either no neurofascin or cytoplasmic-domain truncated neurofascin. B104 neuroblastoma cells were transfected with HA epitope-tagged neurofascin cDNAs (A) and cell segregation experiments were performed as described in the text. Cells expressed all neurofascin constructs to approximately the same level based on immunofluorescence and immunoblot analysis (see Methods). Left panels are images obtained by transmitted light, and right panels are fluorescent images revealing DiA-labeled cells. (B) DiA-labeled cells that were untransfected and express no neurofascin were mixed with unlabeled cells expressing native neurofascin. (C) DiA-labeled cells expressing native neurofascin were mixed with unlabeled cells that express no neurofascin. (D) DiA-labeled cells expressing cytoplasmic domain-truncated neurofascin were mixed with unlabeled cells transfected with native neurofascin. (E) DiA-labeled cells expressing native neurofascin were mixed with unlabeled cells expressing cytoplasmic domain-truncated neurofascin.