Figure 3.

Kinase activity of Gek. Histone phosphorylation by anti-myc immunoprecipitation complex is observed in S2 cells transfected with myc-Gek expression construct (lanes 2 and 4, duplicate experiments), but absent in mock transfected S2 cells (lane 1) as well as in S2 cells transfected with myc-Gek bearing the A105K mutation (lanes 3 and 5, duplicate experiments). The molecular mass marker is shown to the right, and the arrow represents the full-length histone 2A protein (14.5 kDa).