Figure 4.
Involvement of SREBP-binding sites in the androgen regulation of the FAS promoter. A luciferase reporter construct containing a 178-bp promoter fragment of the human FAS gene (FAS-luc) harboring a complex binding site for SREBPs (SREBP-BS) was generated. In the FASmut-luc construct this 18-bp binding site was deleted. LNCaP cells were transfected with 5 μg of these constructs or with 5 μg of a control plasmid containing the simian virus 40 (SV40) promoter (SV40-luc). The next day, the transfected cultures were treated with 10−8 M R1881 (+) or with ethanol vehicle (−). After 24 h cells were harvested and luciferase activity was determined. Luciferase activity was measured in triplicate dishes. Values are the mean ± SD of the indicated number of independent experiments (in parentheses) and are expressed relative to the activity of vehicle-treated cells.