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. 1997 Nov 25;94(24):12981–12986. doi: 10.1073/pnas.94.24.12981

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Copyright © 1997, The National Academy of Sciences of the USA

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Affinity cross-linking of rhCTGF. (A) Autoradiogram of ¹²⁵I-IGF-I (Upper) or ¹²⁵I-IGF-II (Lower) cross-linked to rhCTGF. An approximately 45-kDa band can be seen that is compatible with the 38-kDa rhCTGF bound to 7-kDa ¹²⁵I-IGF-I or ¹²⁵I-IGF-II. (B) Autoradiogram of ¹²⁵I-prolactin cross-linked to 20 pmol of rh-IGFBP-1 (lane 2), rh-IGFBP-3 (lane 3), rh-IGFBP-7 (lane 4) or rhCTGF (lane 5). No binding of prolactin to any of the IGFBPs was observed. The band seen at 33 kDa represents unbound ¹²⁵I-prolactin.