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Northern blot analysis of tissue specificity of indicated androgen-response genes in the rat during hormonal manipulation. N, tissue from the testis-intact rats; −, tissue from 7-day castrated rats; +, tissue from the rats castrated for 7 days followed by androgen replacement for an additional 2 days. The amount and quality of total RNA loaded in the gels were examined by staining the transferred membrane with methylene blue.
