Table 2.
Tissue-specificity survey of androgen-response genes
| Gene | P
|
SV
|
B | Ht | K | Lv | Ms | Ts | ||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| N | C | +T | N | C | +T | |||||||
| U1 | H | – | H | – | – | – | – | – | – | – | – | – |
| U2 | H | – | H | – | – | – | – | – | – | – | – | – |
| U3 | M | – | M | M | M | L | – | – | – | – | – | – |
| U4 | H | – | H | – | – | – | – | – | – | – | – | – |
| U5 | M | – | M | – | – | – | – | – | – | – | – | – |
| U6 | M | – | M | – | – | – | – | – | – | – | – | L |
| U7 | M | L | M | L | L | M | L | L | L | L | L | L |
| U8 | M | L | M | L | L | L | L | L | L | L | L | L |
| U9 | M | L | M | – | – | L | L | L | L | L | L | M |
| U10 | L | – | L | – | – | – | – | – | – | – | – | – |
| U11 | L | – | L | – | – | – | – | – | – | – | – | – |
| U12 | H | L | H | – | – | – | – | – | – | – | – | L |
| U13 | L | – | L | – | – | – | – | – | – | – | – | – |
| U14 | L | – | L | – | – | – | – | – | – | – | – | – |
| U15 | L | – | L | – | – | – | – | – | – | – | – | – |
| U16 | H | – | H | – | – | – | – | – | – | – | – | – |
| U17 | H | L | H | – | – | – | – | – | – | – | – | – |
| U18 | L | – | L | – | – | – | – | – | – | – | – | – |
| U19 | M | L | M | L | – | M | – | – | – | – | – | L |
| U20 | L | – | L | – | – | – | – | – | – | – | – | – |
| U21 | M | L | M | L | L | M | L | L | L | L | L | L |
| U22 | M | L | M | – | – | – | – | – | – | – | – | – |
| U23 | M | L | M | L | L | L | L | L | L | L | L | L |
| U24 | L | – | L | – | – | – | – | – | – | – | – | – |
| U25 | M | – | M | – | – | – | – | – | – | – | – | – |
| D1 | L | H | L | L | H | L | L | L | L | L | L | L |
| D2 | – | L | – | – | – | – | – | – | – | – | – | – |
| D3 | – | L | – | – | – | – | – | – | – | – | – | – |
| D4 | – | L | – | – | – | – | – | – | – | – | – | – |
The abundance of mRNAs was estimated by comparing Northern blot signals with genomic Southern blots as described in Table 1. In the prostate (P) and seminal vesicles (SV) samples, N represents the tissue from the testis–intact rats; C represents the tissue from 7–day castrated rats; and +T represents the tissue from the rats castrated for 7 days followed by testosterone for an additional 2 days. Androgen had no detectable effect on the expression of androgen–response genes in brain (B), heart (Ht), kidney (K), liver (Lv), and muscle (Ms). The expression of androgen–response genes in the testis (Ts) was also determined by Northern blot analysis. High–abundance (H) mRNA is greater than 500 copies per cell; medium–abundance (M) mRNA ranges from 50 to 500 copies per cell; and low–abundance (L) mRNA is about 20 copies per cell. –, mRNA is not detectable with total RNA Northern blot analysis.