Figure 2.

Electrophoretic mobility shift assays showing triplex formation at 37°C in 50 mM Tris–HCl, pH 7.4, 50 mM KCl, 10 mM MgCl₂ and 2 mM spermidine (binding buffer). Experiments were performed with [γ-³²P]ATP-labelled 39-mer murine duplex (5 nM) and 32-mer human duplex (15 nM) of the KRAS gene. Increasing concentrations of TFOs (as indicated) were mixed with the target duplex and incubated overnight in the binding buffer at 37°C. The mixtures were run at 37°C in a 15% native polyacrylamide gel.