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Effect of lithium and potassium on triplex formation at the pur/pyr element of murine KRAS promoter. Oligonucleotides mTFOwt (panel a) and 1037 (panel b), were incubated at 37°C with murine 39-mer duplex (5 nM) in 50 mM Tris–HCl, pH 7.4, 10 mM MgCl2, 2 mM spermidine and 140 mM monovalent cations at various lithium/potassium ratios. The mixtures were incubated overnight at 37°C in the specific buffer and run (at 37°C) in a native 20% polyacrylamide gel.
