Table 1.
Thermodynamic parameters for duplex–triplex equilibrium in 50 mM Tris-HCl, pH 7.4, 50 mM KCl, 10 mM MgCl2, 2 mM spermidine, 37°C, relative to human and murine KRAS duplexes and TINA-TFOs
| Oligonucleotide | Triplex | Kd (M) | ΔG 37 a (kcal/mol) |
|---|---|---|---|
| mTFOwt | No | – | – |
| 1037 | Yes | 8.3 × 10−7 | −8.6 ± 0.4 |
| 1451 | Yes | 3.4 × 10−7 | −9.2 ± 0.2 |
| 1452 | Yes | 2.6 × 10−6 | −7.9 ± 0.2 |
| 1456 | No | – | – |
| hTFOwt | No | – | – |
| 1453 | Yes | 6.1 × 10−7 | −8.8 ± 0.1 |
| 1454 | Yes | 1.3 × 10−7 | −9.8 ± 0.1 |
| 1455 | Yes | 8.7 × 10−7 | −8.6 ± 0.1 |
| 1457 | No | – | – |
aUncertainty estimated by the Marquardt non-linear best-fit program.