Table 1.

Reaction conditions for chloroacetaldehyde probing of extrahelical cytosine residues in enzyme-DNA complexes

Enzyme	CAA concentration range (mM)	Reaction temperature (°C)	Incubation time (min)
M.HhaI	25–200	20–37	15–105
M.AluI	50–200	20–37	50
M.SssI	100–300	20	50
R.Ecl18kI, R.PspGI, R.BcnI	200–1200	37	20–60