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. 2008 Apr 17;36(10):3244–3251. doi: 10.1093/nar/gkn154

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© 2008 The Author(s)

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 4. — Maternal control of IES end cleavage in an IES⁺ cell line. (A) LMPCR detection of DNA double-strand breaks at the left boundary of IES 51G4404 during autogamy of a wild-type IES⁻ cell line (left) and its IES⁺ macronuclear variant (right). Top panels: broken chromosome ends revealed following ligation of linker I′/(GTAT)J′. Middle panels: broken IES ends analyzed with linker I′/(ATAC)J′. Flanking chromosomal sequences are drawn as thin lines, IES sequences as white rectangles and each linker is shown in black. Oligonucleotides are represented by arrows. Bottom panels: broken chromosome ends at the left boundary of control IES 51A2591 analyzed with linker I′/(GTAT)J′. (B) Sequence analysis of band ‘alt’ identified at the left end of IES 51G4404. Bold letters represent the sequence ligated to the linker in the wild-type LMPCR product (‘left’), additional nucleotides found in the ‘alt’ product are in uppercase and upstream flanking sequences in lower case. The positions of the deduced double-strand breaks are shown.