Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2008 Apr 29;36(10):3443–3454. doi: 10.1093/nar/gkn066

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2008 The Author(s)

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Figure 2. — Expression levels of the six selected TRF genes suppressed by respective siRNAs at several time points. HepG2 cells were transfected with each of the specific siRNAs (20 nM, final concentration) 24 h before ATRA addition, and the total RNA was extracted 6, 12, 24, 36 and 48 h after ATRA addition. The expression ratio was calculated according to the 2^−ΔΔC_T method as described in ‘Materials and methods’ section with GAPDH mRNA and negative control siRNA as the internal reference and the calibrator, respectively. The data are the averages of two biological replicates.