Table 2.
ATRA-induced expression changes of genes linked to cell growth, apoptosis and/or cell cycle
| Symbol | RefSeq ID | 6 h | 12 h | 24 h | 36 h | 48 h |
|---|---|---|---|---|---|---|
| ANXA3* | NM_005139 | 2.56 | 2.83 | 2.24 | ||
| AREG | NM_001657 | 3.88 | ||||
| BHD | NM_144606 | 2.50 | ||||
| BMP4 | NM_130851 | 0.44 | ||||
| C20orf97 | NM_021158 | 2.51 | 3.12 | 3.84 | ||
| CARD9 | NM_052814 | 2.63 | 2.48 | |||
| CCND2 | NM_001759 | 2.09 | ||||
| CNK | NM_004073 | 3.52 | ||||
| CTGF | NM_001901 | 0.46 | ||||
| DDIT3 | NM_004083 | 2.22 | 2.01 | 2.38 | 4.09 | 3.98 |
| DTNA | NM_001392 | 2.26 | ||||
| DUSP1 | NM_004417 | 2.69 | 2.65 | |||
| DUSP6 | NM_022652 | 0.42 | 0.37 | 0.38 | ||
| ELF5 | NM_001422 | 0.40 | ||||
| FABP3 | NM_004102 | 0.42 | ||||
| FGA | NM_021871 | 2.37 | 2.44 | |||
| FGB | NM_005141 | 2.55 | 3.44 | 2.36 | ||
| FGG | NM_000509 | 2.44 | 2.06 | 2.08 | ||
| FLJ30999 | NM_152461 | 2.94 | ||||
| FLJ31051 | NM_153687 | 0.43 | ||||
| FSCN1 | NM_003088 | 0.39 | ||||
| FSTL3 | NM_005860 | 4.99 | 3.80 | 4.30 | 7.88 | 9.95 |
| GPC6 | NM_005708 | 2.26 | 2.14 | 2.82 | ||
| HYPE | NM_007076 | 2.91 | 2.30 | |||
| IFITM1 | NM_003641 | 0.41 | 0.29 | |||
| IGF2 | NM_000612 | 2.39 | 2.09 | 4.50 | 5.11 | 9.80 |
| IGFBP1 | NM_000596 | 2.27 | ||||
| IGFBP6 | NM_002178 | 2.15 | ||||
| IGFBP7 | NM_001553 | 0.48 | 0.33 | |||
| IL17RB | NM_018725 | 0.43 | ||||
| INHBE | NM_031479 | 2.94 | 6.65 | 5.63 | 4.71 | |
| LIF | NM_002309 | 4.69 | ||||
| LOC117584 | NM_057178 | 0.47 | ||||
| MAPK13 | NM_002754 | 2.36 | ||||
| MDK | NM_002391 | 2.37 | ||||
| MGC8685 | NM_178012 | 3.24 | 3.78 | 4.38 | ||
| MLLT3 | NM_004529 | 0.45 | ||||
| MLLT7 | NM_005938 | 0.49 | ||||
| MXD4 | NM_006454 | 0.36 | ||||
| NEDD9 | NM_182966 | 0.40 | ||||
| OKL38 | NM_182981 | 2.40 | 2.23 | |||
| PDZK1 | NM_002614 | 0.47 | 0.45 | 0.31 | 0.23 | |
| PEG10 | NM_015068 | 0.36 | 0.29 | |||
| PLAB | NM_004864 | 2.72 | 2.45 | |||
| PLAU | NM_002658 | 0.43 | 0.38 | 0.34 | ||
| PPP1R15A | NM_014330 | 3.66 | 3.87 | |||
| RARA | NM_000964 | 2.21 | 2.65 | |||
| SESN2 | NM_031459 | 2.29 | 2.39 | 2.85 | ||
| SGK | NM_005627 | 0.33 | 0.23 | 0.18 | 0.26 | |
| TGM2* | NM_198951 | 2.24 | 2.25 | |||
| TNFRSF12A | NM_016639 | 2.70 | ||||
| TNFRSF19 | NM_018647 | 0.34 | ||||
| TNFSF10 | NM_003810 | 0.42 | 0.46 | |||
| TUBE1 | NM_016262 | 2.66 | 2.88 | |||
| WARS | NM_004184 | 2.40 |
The expression ratio represents the ratio of the expression level in the cells treated with ATRA to that in those incubated with only DMSO for the periods of time indicated. The four profiling data sets (two biological and two technical replicates) were categorized into three groups: each of the biological replicates and a sum of all the four replicates. Only data exhibited a P-value <0.01 were extracted and only genes whose expression levels were significantly changed in two or all of the three groups were selected. Results are the average of two or three groups of data analyzed. Genes with an asterisk were not assigned to any GO category, but are known to be related to cell growth or apoptosis (34,35).