Table 2.
Apparent melting temperatures of HP25 hairpin substituted with different G-C couplesa
| LNA–DNA probe | DNA probe | ||||
|---|---|---|---|---|---|
| Couple | Tm (°C) | bPC | Couple | Tm (°C) | bPC |
| G-C | 76 | – | |||
| IcG-MeC | 67 | 0.47 | cG-MeC | 65 | 0.61 |
| cG-MeC | 66 | 0.56 | cG-EtC | 57 | 1.05 |
| cG-EtC | 64 | 0.67 | IcG-MeC | 52 | 1.26 |
| EtcG-MeC | 62 | 0.93 | IcG-EtC | 50 | 1.37 |
| IcG-EtC | 56 | 1.05 | EtcG-MeC | 50 | 1.73 |
| EtcG-EtC | 53 | 1.53 | MecG-MeC | 55 | 1.75 |
| MecG-MeC | 56 | 1.67 | EtcG-EtC | 42 | 2.27 |
| MecG-EtC | 55 | 1.75 | MecG-EtC | 46 | 2.50 |
aTwo different 25-mer probes were used to determine the apparent melting temperatures of HP25 hairpins substituted with the indicated G-C couples. bThe PC ratio is a measure of the extent to which a G-C couple is pseudo-complementary. PC = (ΔTm of HP25 with indicated G-C couple)/(ΔTm of HP25 with G analog only + ΔTm of HP25 with C analog only).