Table 3.
Apparent melting temperatures of HP25 hairpin substituted with different G-C couples in the presence of nA and sTa
| Couple | G | IcG | cG | MecG | EtcG |
|---|---|---|---|---|---|
| mcvC | 42 | 52 | 39 | 38 | 41 |
| cnC | – | 38 | 41 | 41 | 32 |
| fC | 44 | 40 | 38 | 37 | 36 |
| C | 40 | 36 | 26 | 27 | 18 |
| MeC | 28 | 29 | 12 | <10 | <10 |
| EtC | 25 | <10 | <10 | <10 | <10 |
aTemperature in °C.