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. 2008 Apr 24;36(10):3297–3310. doi: 10.1093/nar/gkn184

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© 2008 The Author(s)

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 1. — DNA substrates of end-joining assays. (A) The plasmid pUC18PD1/4 was digested with Eco47III and EcoRV, resulting in a blunt-ended linear molecule with a 10-bp direct repeat (ATCCTACAGC) at each end. Deletion of one 10-bp repeat in joined DNA will produce an XcmI restriction site. This particular restriction site can not be created by other joining reactions. (B) The plasmids pUC18-2-bp-repeat and pUC18-3-bp-repeat were digested with PshAI, resulting in blunt-ended linear molecules with a 2-bp (AG) or 3-bp (CAG), respectively, direct repeat at each end.