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Requirement of DNA ligase III in MHEJ. (A) The effect of anti-ligase III antibodies. Nuclear extract from HTD114 cells (1.6 µg total protein) was incubated for 30 min on ice with the antibody against human DNA ligase III, then incubated with 400 ng linear DNA at 14°C. After 1 h incubation, DNA products were analyzed by agarose gel electrophoresis. (B) PCR products of end-joined products shown in (A) were digested with XcmI. (C) Quantification of MHEJ.
