Table 2.

Accuracy of prediction for SH2-binding peptides by SMALI or Scansite^a

SH2 domain	SMALI score cut-off	SMALI		Scansite
		SMALI score (average, SD)	Hit rate (%)	SMALI score (average, SD)	Hit rate (%)
NCK1	1.40	2.02, 0.11	40	1.73, 0.29	15
CRK	1.65	2.19, 0.08	90	1.64, 0.28	32
FGR	1.35	1.84, 0.10	98	1.49, 0.30	87

^aPeptides with spot values >0.8 are defined as binding peptides for the NCK1 SH2 domain, >0.7 for the CRK SH2 domain and >0.4 for FGR SH2 domain, based on the distribution of spot values in a peptide array experiment (see Materials and Methods section for details; see also Figure 4 and Tables S3–S5 for experimental data).