Figure 1.

Schematic representation of the Blast2GO application. GO annotations are generated through a three-step process: BLAST, mapping, annotation. InterPro terms are obtained from InterProScan at EBI, converted and merged to GOs. GO annotation can be modulated from Annex, GOSlim web services and manual editing. Enzyme Code and KEGG Pathway map annotations are retrieved through mappings from GO. Visual tools include sequence colour code, KEGG pathways and GO graphs with GO term highlighting and filtering options. Additional annotation data-mining tools include statistical charts and gene set enrichment analysis functions.