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. 2008 Apr 25;36(10):3341–3353. doi: 10.1093/nar/gkn208

Figure 1.

Figure 1.

Screening for the modulation of transcription factor DNA binding using TranSignal protein/DNA array I. (A) Structure of DB293 compound. (B–D) Membrane of protein DNA/array I. The mixture of TranSignal biotinylated oligonucleotides was incubated with HT-29 nuclear extracts in the absence (B, control) or presence of 1 µM (C) or 5 µM (D) of DB293. (E) Inhibition ratio for the transcription factor/DNA-binding activities induced by DB293. The DNA-binding activity of these transcription factors is specified: ‘ATGA’ corresponds to a consensus-binding sequence containing an ATGA site and ‘other’ to a consensus-binding site that contains neither an ATGA nor an AT-rich site. The values correspond to the ratio between the analyses performed in the presence or absence of the drug, after normalization of the points relative to the internal controls (right and bottom lanes in Figure 1B–D).