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. 2008 Apr 25;36(10):3341–3353. doi: 10.1093/nar/gkn208

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© 2008 The Author(s)

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 3. — DNaseI footprinting of DB293 on Pit-1, Brn-3 and IRF-1 consensus-binding sites. The concentration (µM) of the drug is shown at the top of the appropriate lanes. Control tracks labeled ‘DNA’ contained no drug. Tracks labeled ‘G’ represent dimethyl sulfate-piperidine-treated DNA sample exemplifying guanines location. The cloned sequences containing the various consensus-binding sites in the context of that used in the TranSignal DNA array approach are located between two arrows (). Pit-1 cloned site is inverted in comparison with that for Brn-3 and IRF-1 consensus-binding sites. The localization of the footprints is specified using black boxes on the gels (A–C) and on the respective densitometric analysis (D–F). The plots are expressed as the ln (0.5, 1, 1.5 or 2 µM) of DB293/control DNA alone.

Inline graphic — DNaseI footprinting of DB293 on Pit-1, Brn-3 and IRF-1 consensus-binding sites. The concentration (µM) of the drug is shown at the top of the appropriate lanes. Control tracks labeled ‘DNA’ contained no drug. Tracks labeled ‘G’ represent dimethyl sulfate-piperidine-treated DNA sample exemplifying guanines location. The cloned sequences containing the various consensus-binding sites in the context of that used in the TranSignal DNA array approach are located between two arrows (). Pit-1 cloned site is inverted in comparison with that for Brn-3 and IRF-1 consensus-binding sites. The localization of the footprints is specified using black boxes on the gels (A–C) and on the respective densitometric analysis (D–F). The plots are expressed as the ln (0.5, 1, 1.5 or 2 µM) of DB293/control DNA alone.