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. 2008 Apr 25;36(10):3341–3353. doi: 10.1093/nar/gkn208

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© 2008 The Author(s)

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 7. — Electrophoretic mobility shift assays for the binding of Pit-1 transcription factor on wild-type or mutated consensus-binding sequence. (A) Radiolabeled WT or M1 to M6 Pit-1 oligonucleotides specified on the top of the lanes were incubated alone or with HT-29 nuclear extracts. Full and open arrows are as specified in Figure 2. Nonspecific (NS) protein/DNA complexes, localized using an asterisk (*), are observed using those oligonucleotides which are longer than that used for Figure 2 and were identified using a 50-fold concentration of specific or NS nonlabeled probes relatively to the radiolabeled specific DNA probe (data not shown). (B) The central part of the various sequences is presented with arrows exemplifying the importance of the relative mutation points.