Table 2.
Primers used and estimated product length
| Gene | GeneID | Primers (5′ → 3′) | CORE index | Size | Location (human) | |
|---|---|---|---|---|---|---|
| AOF2 | 23028 | F | ATGCAGTTCTCTGTACCCTTCC | 41 | 550 | Exon 15–Exon 16 |
| R | AACATGCCCNAACAAATTGAC | |||||
| EFEMP1 | 2202 | F | GCATTGCAAAACTCTGTATGG | 37 | 650 | Intron 6–Exon 7 |
| R | TACCTTCACAGTTGAGCCTGTC | |||||
| LRP6 (1) | 4040 | F | ATCAGNTCCCTCAGTATCATGG | 37 | 800 | Exon 21–Exon 22 |
| R | TAATGTGATCGCTCTGTGG | |||||
| LRP6 (2) | 4040 | F | GAACTCAATTGTCCTGTNTGCTC | 37 | 1200 | Exon 18–Exon 19 |
| R | CAGTTCATCTGANTTGTCACTGC | |||||
| OAZ1 | 4946 | R | TCCCTNCACTGCTGTAGTAACC | 33 | 550 | Exon 2–Exon 3 |
| F | CNGGGATCTCGATGTAGAGG |
For each gene/locus the forward (F) and reverse (R) primers are indicated. The ‘GeneID’ was the entry used for the initial step. The ‘CORE index’ has been provided by T-coffee, and is the reliability score of the alignments. The expected product length is an average value inferred from the multiple alignment but varies between species. Two sets of primers were used for the LRP6 gene.