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Effect of alternative sequences on unwinding by Srs2 and Sgs1. (A) The unwinding of duplex containing (CTT)7, a nonstructure forming loop. (B) The unwinding of duplexes with (TCCTGG)3 structured loop substrate. (C and D) Quantification of helicase activity on (CTT)7 (C) and the structured loop substrate (D). Filled squares denote Srs2 and unfilled squares represent Sgs1. The shorter strand is radiolabeled on the 5′ end, as denoted by the asterisk.
