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. 2008 Apr 27;36(10):3374–3388. doi: 10.1093/nar/gkn108

Table 1.

New plasmids used in this study

Plasmid Description
pHD1534 DHH1 RNAi Part of the DHH1 ORF was amplified using forward primer 5′-gag aag atc taa gct tca cag tca agg act ttg cg-3′and reverse primer 5′-cgg aat tcg tcg acc caa tgc ggt gaa ggt aag t-3′, PCR product was cloned into p2T7TA blue (39)
pHD1535 LCCR4 RNAi Part of the CCR4-like ORF was amplified using forward primer 5′-gagaagatctgcatgccaaagcaatctgtggaagca-3′ and reverse primer 5′-cggaattcgtcgacccaaccaccaactcttcgtt-3′, PCR product was cloned into p2T7TA blue (39)
pHD1843 CAF1 RNAi Part of the CAF1 ORF was amplified using forward primer 5′-gaggatcsctctgcctcacagaggatgtg-3′ and reverse primer 5′-cgggatcccgaggaatcttcacagagcc-3′, PCR product was cloned into p2T7TA blue (39)
pHD1844 NOT1 RNAi Part of the NOT1 ORF was amplified using forward primer 5′-gaggatccgagctggtagaagtggacgc-3′ and reverse primer 5′-cgggatccacgactcgggaccacaatag-3′, PCR product was cloned into p2T7TA blue (39)
pHD1847 GST-CAF1 CAF1 ORF was amplified using forward primer 5′-gagagaattcatgcagtatggtggcgggac-3′ and reverse primer 5′-cgatgaattctcagctatgaccctttaccgc-3′ and cloned into pGEX-λ T (Eco RI digest)
pHD1849 CAF1-TAP tag (C-terminal) CAF1 ORF was amplified using forward primer 5′-aagcttatgatgcagtatggtggcggga-3′ and reverse primer 5′-gttaacgctatgaccctttaccgcttgtgt-3′and cloned into pHD 924 (Hind III, Hpa I digest) (34)
pHD1850 LCCR4- TAP tag (C-terminal) LCCR4 ORF was amplified using forward primer 5′-aagcttatggcacggcaccccaacacggc-3′ and reverse primer 5′-gttaactttgtacgacaaatcggcaatcaa-3′ and cloned into pHD 1337 (Hind III, Hpa I digest) (34)