Table 1.
New plasmids used in this study
| Plasmid | Description | |
|---|---|---|
| pHD1534 | DHH1 RNAi | Part of the DHH1 ORF was amplified using forward primer 5′-gag aag atc taa gct tca cag tca agg act ttg cg-3′and reverse primer 5′-cgg aat tcg tcg acc caa tgc ggt gaa ggt aag t-3′, PCR product was cloned into p2T7TA blue (39) |
| pHD1535 | LCCR4 RNAi | Part of the CCR4-like ORF was amplified using forward primer 5′-gagaagatctgcatgccaaagcaatctgtggaagca-3′ and reverse primer 5′-cggaattcgtcgacccaaccaccaactcttcgtt-3′, PCR product was cloned into p2T7TA blue (39) |
| pHD1843 | CAF1 RNAi | Part of the CAF1 ORF was amplified using forward primer 5′-gaggatcsctctgcctcacagaggatgtg-3′ and reverse primer 5′-cgggatcccgaggaatcttcacagagcc-3′, PCR product was cloned into p2T7TA blue (39) |
| pHD1844 | NOT1 RNAi | Part of the NOT1 ORF was amplified using forward primer 5′-gaggatccgagctggtagaagtggacgc-3′ and reverse primer 5′-cgggatccacgactcgggaccacaatag-3′, PCR product was cloned into p2T7TA blue (39) |
| pHD1847 | GST-CAF1 | CAF1 ORF was amplified using forward primer 5′-gagagaattcatgcagtatggtggcgggac-3′ and reverse primer 5′-cgatgaattctcagctatgaccctttaccgc-3′ and cloned into pGEX-λ T (Eco RI digest) |
| pHD1849 | CAF1-TAP tag (C-terminal) | CAF1 ORF was amplified using forward primer 5′-aagcttatgatgcagtatggtggcggga-3′ and reverse primer 5′-gttaacgctatgaccctttaccgcttgtgt-3′and cloned into pHD 924 (Hind III, Hpa I digest) (34) |
| pHD1850 | LCCR4- TAP tag (C-terminal) | LCCR4 ORF was amplified using forward primer 5′-aagcttatggcacggcaccccaacacggc-3′ and reverse primer 5′-gttaactttgtacgacaaatcggcaatcaa-3′ and cloned into pHD 1337 (Hind III, Hpa I digest) (34) |