Table 3.
Mass spectrometry of proteins associated with TAP-tagged CAF1
Band | GeneDB number | Protein function (known or putative) | Score | Peptides | Coverage | Molecular weight | Migration |
---|---|---|---|---|---|---|---|
1 | Tb10.70.6450 | NOT1 | 3389 | 65 | 33 | 259 | >220 |
2 | Tb927.2.280 (and related)a | Retroposon hotspot protein (several similar predicted proteins) | 251 | 7 | 8 | 91 | 90-100 |
2 | Tb11.02.1070 | Aminopeptidase | 57 | 3 | 3 | 98 | 100 |
3 | Tb927.3.1920 | NOT3/5 | 815 | 19 | 29 | 67 | 85 |
3 | Tb11.02.1600 | Ubiquitin-like | 92 | 2 | 3 | 67 | 85 |
4 | Tb10.6k15.1820 | TPR repeat protein, similar to CNOT10/Caf130 | 578 | 11 | 21 | 61 | 65 |
4 | Tb11.02.1860 | Hypothetical conserved, PF02622, domain of unknown function | 105 | 2 | 4 | 52 | 65 |
5 | Tb927.1.2340a | Alpha tubulin | 975 | 20 | 5 | 50 | 50 |
5 | Tb927.1.2330a | Beta tubulin | 614 | 14 | 28 | 50 | 50 |
6 | Tb927.6.600 | CAF1 | 196 | 7 | 16 | 39 | 45 |
6 | Tb927.6.850 | NOT2 | 172 | 4 | 8 | 37 | 45 |
6 | Tb10.70.3290 | DHH1 | 52 | 2 | 4 | 46 | 45 |
6 | Tb09.211.0620 | Actin A | 135 | 3 | 11 | 42 | 45 |
6 | Tb927.8.1960 | Hypothetical conserved | 714 | 20 | 43 | 48 | 45 |
The overall score, number of different peptides identified, and % coverage of the amino acid sequence are shown.
aBands 2 and 5 predominantly consisted of proteins found frequently as contaminants.