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. Author manuscript; available in PMC: 2009 Apr 1.
Published in final edited form as: Dev Biol. 2008 Jan 26;316(1):74–86. doi: 10.1016/j.ydbio.2008.01.011

Figure 6.

Figure 6

Early endocrine cells develop independently of Pdx-1 and Ptf1a. Immunofluorescence analysis for insulin, glucagon and Pdx-1. A) First wave insulin-and glucagon-expressing cells are associated with the pancreatic epithelium of wild type mice at E12.5. These endocrine cells are also present in the DPR of Pdx-1 null (B), and Pdx-1; Ptf1a double null (C) mice which indicates that neither transcription factor is necessary for their formation. D) Ngn3 is observed in the dorsal pancreas of wild type embryos at E12.5 but not in Pdx-1 null (E) or Pdx-1; Ptf1a double null (F) embryos, which suggests that Pdx-1 is required to sustain the specification of pancreatic Ngn3–expressing endocrine progenitors from an MPC pool. Scale bars = 25 µm.

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