Table 1.
Interaction of various ligands with the cysteine-rich domain of the IGF-1 receptor family
| GAL4DB hybrid | GAL4AD hybrid | Colony color | β-Galactosidase activity, units |
|---|---|---|---|
| Empty | Empty | − | <1 |
| Empty | IGF1R(1–315) | − | 2 |
| IGF-1 | Empty | − | |
| IGF-1 | IGF1R(1–315) | +++ | 320 |
| proIGF-1 | Empty | − | 11 |
| proIGF-1 | IGF1R(1–315) | +++ | 328 |
| Proinsulin | Empty | − | |
| Proinsulin | IGF1R(1–315) | ± | 29 |
| proIGF-1 | IRR(1–302) | − | <1 |
The intensity of the interaction was measured either in the colony color assay or as β-galactosidase activity as described in the legend of Fig. 1. The cysteine-rich domain of the IGF-1 receptor is denoted IGFR(1–315) and that of the IRR as IRR(1–302). “Empty” indicates no insert in the vector.