Skip to main content
Applied and Environmental Microbiology logoLink to Applied and Environmental Microbiology
. 1977 Sep;34(3):280–284. doi: 10.1128/aem.34.3.280-284.1977

Thermal inactivation of ileal loop-reactive Clostridium perfringens type A strains in phosphate buffer and beef gravy.

J G Bradshaw, J T Peeler, R M Twedt
PMCID: PMC242643  PMID: 199113

Abstract

The thermal resistance of spore crops produced from each of two ileal loop-reactive strains of Clostridium perfringens type A was determined in two suspending vehicles consisting of 0.067 M (pH 7.0) phosphate buffer and a commercial beef gravy. D115.6 values obtained in buffer and enumerated after pretreatment with sodium ethylenediaminetetraacetate and recovery in plating medium containing lysozyme were two- to threefold greater than those obtained without this treatment. D115.6 values obtained with beef gravy were less than those obtained in buffer with or without lysozyme; however, the D98.9 and D104.4 values were 1.3 to 2 times greater than those obtained in buffer with lysozyme. The z values were within the ranges reported by previous investigators.

Full text

PDF
280

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Adams D. M. Inactivation of Clostridium perfringens type A spores at ultrahigh temperatures. Appl Microbiol. 1973 Sep;26(3):282–287. doi: 10.1128/am.26.3.282-287.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Adams D. M. Requirement for and sensitivity to lysozyme by Clostridium perfringens spores heated at ultrahigh temperatures. Appl Microbiol. 1974 Apr;27(4):797–801. doi: 10.1128/am.27.4.797-801.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Alderton G., Chen J. K., Ito K. A. Effect of lysozyne on the recovery of heated Clostridium botulinum spores. Appl Microbiol. 1974 Mar;27(3):613–615. doi: 10.1128/am.27.3.613-615.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Drucker M. M., Yeivin R., Sacks T. G. Pathogenesis of Escherichia coli enteritis in the ligated rabbit gut. Isr J Med Sci. 1967 May-Jun;3(3):445–452. [PubMed] [Google Scholar]
  5. Duncan C. L., Labbe R. G., Reich R. R. Germination of heat- and alkali-altered spores of Clostridium perfringens type A by lysozyme and an initiation protein. J Bacteriol. 1972 Feb;109(2):550–559. doi: 10.1128/jb.109.2.550-559.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Duncan C. L., Strong D. H. Clostridium perfringens Type A Food Poisoning I. Response of the Rabbit Ileum as an Indication of Enteropathogenicity of Strains of Clostridium perfringens in Monkeys. Infect Immun. 1971 Jan;3(1):167–170. doi: 10.1128/iai.3.1.167-170.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Duncan C. L., Strong D. H. Improved medium for sporulation of Clostridium perfringens. Appl Microbiol. 1968 Jan;16(1):82–89. doi: 10.1128/am.16.1.82-89.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Duncan C. L., Sugiyama H., Strong D. H. Rabbit ileal loop response to strains of Clostridium perfringens. J Bacteriol. 1968 May;95(5):1560–1566. doi: 10.1128/jb.95.5.1560-1566.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Genigeorgis C. Public health importance of Clostridium perfringens. J Am Vet Med Assoc. 1975 Nov 1;167(9):821–827. [PubMed] [Google Scholar]
  10. HALL H. E., ANGELOTTI R. CLOSTRIDIUM PERFRINGENS IN MEAT AND MEAT PRODUCTS. Appl Microbiol. 1965 May;13:352–357. doi: 10.1128/am.13.3.352-357.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. HALL H. E., ANGELOTTI R., LEWIS K. H., FOTER M. J. CHARACTERISTICS OF CLOSTRIDIUM PERFRINGENS STRAINS ASSOCIATED WITH FOOD AND FOOD-BORNE DISEASE. J Bacteriol. 1963 May;85:1094–1103. doi: 10.1128/jb.85.5.1094-1103.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Harmon S. M., Kautter D. A., Peeler J. T. Improved medium for enumeration of Clostridium perfringens. Appl Microbiol. 1971 Oct;22(4):688–692. doi: 10.1128/am.22.4.688-692.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Hauschild A. H., Niilo L., Dorward W. J. Clostridium perfringens type A infection of ligated intestinal loops in lambs. Appl Microbiol. 1968 Aug;16(8):1235–1239. doi: 10.1128/am.16.8.1235-1239.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Hauschild A. H., Niilo L., Dorward W. J. The role of enterotoxin in Clostridium perfringens type A enteritis. Can J Microbiol. 1971 Jul;17(7):987–991. doi: 10.1139/m71-156. [DOI] [PubMed] [Google Scholar]
  15. Roberts T. A. Heat and radiation resistance and activation of spores of Clostridium welchii. J Appl Bacteriol. 1968 Mar;31(1):133–144. doi: 10.1111/j.1365-2672.1968.tb00350.x. [DOI] [PubMed] [Google Scholar]
  16. Shahidi S. A., Ferguson A. R. New quantitative, qualitative, and confirmatory media for rapid analysis of food for Clostridium perfringens. Appl Microbiol. 1971 Mar;21(3):500–506. doi: 10.1128/am.21.3.500-506.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Strong D. H., Duncan C. L., Perna G. Clostridium perfringens Type A Food Poisoning II. Response of the Rabbit Ileum as an Indication of Enteropathogenicity of Strains of Clostridium perfringens in Human Beings. Infect Immun. 1971 Jan;3(1):171–178. doi: 10.1128/iai.3.1.171-178.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Applied and Environmental Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES