Figure 5.
Subcellular localization of APOBEC-1 in apoB RNA-editing-competent versus incompetent cell lines. (A) HA-APOBEC-1 was expressed and localized in McArdle (A1), HepG2 (A2), CHO (A3), and COS-7 (A4) cells by immunofluorescence analysis. Insets correspond to DAPI-stained fields indicating the location of the cell nucleus. The transfection efficiencies were as follows: McArdle, 15% ± 6%, n = 4; HepG2, 4% ± 2%, n = 5; CHO 7% ± 2%, n = 4; COS-7 23% ± 7%, n = 4. Two representative fields for each transfection are shown. (B) An equal amount of extracts from 105 cells of each cell line was used to evaluate the expression level of APOBEC-1 by immunoblotting analysis.