Figure 1.

TPE is reduced in cac1Δ cells. Strains with URA3 at the chromosome VII-L telomere were grown at 30°C and assayed for TPE by plating 10-fold serial dilutions of two independent colonies for each strain on FOA medium. Dilutions were also plated on YC–Ura medium to determine the total number of cells. Two independent platings are shown in Upper and Lower to illustrate the variation typically observed for cac1Δ cells. Photographs were taken after the indicated number of days. WT, wild type.