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A telomeric ADE2 gene is either “on” or “off” in cac1Δ cells. TPE was assayed using a color marker, the ADE2 gene, at the chromosome V-R telomere (34). Colonies were plated on YC medium with a low adenine concentration (10 mg/liter), allowed to grow at 30°C for 3 days, and then placed at 4°C for approximately 3 weeks to allow the red color to develop.
