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CBS is virtually eliminated in cac1Δ cells. Wild-type and cac1Δ strains that contain a 1-kb tract of internal C1–3A sequence adjacent to a URA3 gene at the LYS2 locus (see map at the top) were assayed for CBS by plating 10-fold serial dilutions of cells on FOA and YC-Ura plates and incubating for 3 or 5 days at 30°C.
