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. 1997 Nov 25;94(24):13081–13086. doi: 10.1073/pnas.94.24.13081

Table 1.

Growth of individual cells after micromanipulation

No. of divisions FOA to FOA URA3-TEL
FOA to YC URA3-TEL
FOA to FOA ura3-52
Wild type
cac1Δ
Wild type
cac1Δ
Wild type
cac1Δ
% No. % No. % No. % No. % No. % No.
0 42 198 62 244 8 19 8 15 2 2 6 5
1–2 8.5 38 15 57 4 9 0.5 1 0 0 0 0
3 or more 49 204 23 84 88 198 91 169 97 79 94 75
Total cells 100 440 100 385 100 226 100 185 100 81 100 80
Stops* 16% 40% 4% 0.6% 0% 0%

The percentage of cells in each growth category is presented; No. indicates the total number of cells analyzed. 

*

The percentage of cells that stopped dividing after 1–2 divisions of the total cells that divided is presented. For example, in the first column, of the 242 (38 + 204) cells that started to divide, 38 or 16% of them stopped after 1–2 divisions. A χ2 test using the summed totals of the number of cells in each of the two categories (1–2 divisions and 3 or more divisions) for wild-type URA3-TEL and cac1Δ URA3-TEL, FOA to FOA, indicated that the difference between 16% and 40% is significant. χ2 = 16.864, P < 0.0001.