Table 1.
No. of divisions | FOA to FOA URA3-TEL
|
FOA to YC URA3-TEL
|
FOA to FOA ura3-52
|
|||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
Wild type
|
cac1Δ
|
Wild type
|
cac1Δ
|
Wild type
|
cac1Δ
|
|||||||
% | No. | % | No. | % | No. | % | No. | % | No. | % | No. | |
0 | 42 | 198 | 62 | 244 | 8 | 19 | 8 | 15 | 2 | 2 | 6 | 5 |
1–2 | 8.5 | 38 | 15 | 57 | 4 | 9 | 0.5 | 1 | 0 | 0 | 0 | 0 |
3 or more | 49 | 204 | 23 | 84 | 88 | 198 | 91 | 169 | 97 | 79 | 94 | 75 |
Total cells | 100 | 440 | 100 | 385 | 100 | 226 | 100 | 185 | 100 | 81 | 100 | 80 |
Stops* | 16% | 40% | 4% | 0.6% | 0% | 0% |
The percentage of cells in each growth category is presented; No. indicates the total number of cells analyzed.
The percentage of cells that stopped dividing after 1–2 divisions of the total cells that divided is presented. For example, in the first column, of the 242 (38 + 204) cells that started to divide, 38 or 16% of them stopped after 1–2 divisions. A χ2 test using the summed totals of the number of cells in each of the two categories (1–2 divisions and 3 or more divisions) for wild-type URA3-TEL and cac1Δ URA3-TEL, FOA to FOA, indicated that the difference between 16% and 40% is significant. χ2 = 16.864, P < 0.0001.